Protein Analysis                                     


Search literature, nucleotide, protein, structural, genome, genes in disease(OMIN)databases and moreA collection of sites for research funding resourcesA collection of sites for research funding resources

A good web search engine
 
Posttranslational modifications
RESID predicts postranslational modifications
NetPhos at Expasy  and CBS predicts phosphorylation sites
O- glycosylation sites and NetOglyc
GPI anchor  and big-PI Predictor- GPI Modification Site Prediction
SIGNAL PEPTIDE PREDICTION and Signalp V1.1 and SignalP and PSort
PredictProtein  and TMpred predict transmembrane spanning domains and topology
 
Multiple sequence alignment

Ensemble for finding orthologues in genomes

Protein Domain motif Searches
Pfam _ HMM Search (St. Louis)
Expasy has one of the best collection of pattern and profile search tools
SMART - Simple Modular Architecture Research Tool
BLOCKS is a protein motif database
The ProDom protein domain database
pattern scanner
Hits at Expasy- Relationships between protein sequences and motifs
Prosite and PPSEARCH is a database of protein families and domains
PRINTS is a protein motif fingerprint database
Frame-ProfileScan -at Expasy Scans a short DNA sequence against protein databases
DOMO is a protein domain database
Proteome sites
Proteome Inc has great annotated database to Yeast and worms
GadFly and FlyBase have annotated fly database
 
Protein Families
Proweb is one of the best links for protein families
MEROPS is a protease database
G-protein coupled receptors
Protein kinase resource has sequence alignments, structural information, techniques, diseases, and more resources concerning protein kinases.
Protein families at CMS has more than 25 different protein families
Protein families at PKR has web pages for more than 15 different protein families
 
Protein structure
RasMol software for viewing 3-dimensional structures
Swiss-model will model a 3 dimensional structure based on homology to other proteins
BioMagRsBank is a depository of NMR data
UCONN-tools for NMR and other tools for protein structure
MolSurfer is a useful program for looking at surface interactions between two proteins
WebMol displays and analyzes structural information
PDB is a structural database

NMR LINKS is a jumpstation for links to NMR resources

DALI

for looking for proteins with similar 3D structures
 
Protein sorting signals and specific motifs
Leucine zippers predicts leucine zippers
PEST at Expasy and PESTfind- Identification of PEST regions
PSORT and PSORT2 identifies protein sorting signals
 
Protein Physics
Peptide Mass predicts mass from a nucleotide sequence
ExPASy - ProtParam tool computes PI, aliphatic index, composition ect.
ProtScale assigns numerical scale to amino acids in proteins based on physical properties
hydropathy plots generates a GIF output hydropathy plot
Keck Facility for mass spec and biophysics

MOTIF SITES

List of motif sites

 

Yeast two-hybrid screens
Yeast tools - menu
False positives in Interaction Trap Table of false positives
 
Utilities
Convert  changes sequence formats
ExPASy - Translate tool for translating DNA sequences
BoxShade Server make colored format of multiple sequence alignments
ORF_ DNA Sequence Translation translates nucleotide sequence and finds open reading frames
Genewise at Expasy will compare a protein sequence to genomic sequence allowing for introns
Enzyme nomenclature database
Mass-spec protein resources at Expasy

 

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